I have some pink spawn which was being expanded out in jars of rye berries and popcorn kernels. There’s been good growth and since a couple weeks ago the jar appeared to be completely colonized (at which time I left one jar sitting and moved another jar to a couple fruiting bags). It’s been sitting on the basement floor since then, but I’ve noticed some pink coloration that seems to have gone throughout the jar, and there seems to be a bit of the pink also in the fruiting bags.
I prepared some jars of blue oyster spawn at the same time, using the same batch of rye and popcorn, and those jars are still snow white. This has me wondering, could I have possibly gotten contamination ONLY in the pink oyster jars while all of the blue oyster jars somehow escaped contamination? Or is this pink color natural for pink oysters? It just seems like an awful big coincidence that only the pink oysters would get contaminated when everything was prepped together in the still-air box and there was definitely cross-exposure between the jars.
Since this is my first batch of oysters I’m just not sure what I should be expecting.
FYI I updated the original post with a pic of the jar, plus a link to the full-sized pic.
Thanks! The myc looks totally fine! :)
Yesterday, when I looked up mine, it looked white, but it will discolor soon. The pink oysters produce pink metabolites and knots when more mature, especially when contained, when moisture can build up.
Yeah I’m getting a bit concerned about the moisture as well. These last three jars were set aside to use for the next run of fruiting bags, but they’re getting a lot of moisture built up inside the jar. I was going to put them in the fridge when they reached a good point but someone told me that was a bad idea, and that was a couple weeks ago. I don’t know how long they can sit at room temperature but it’s probably going to be another month before I’m ready to use them.
Good to know about the color, though! I’ve noticed the pink oysters seem to take a lot longer to colonize than the blue oysters do so I’m expecting my fruiting bags to take twice as long as the blues I set up at the same time (and one of the blues is going absolutely crazy!).
I wouldn’t say they take longer. Oysters in general don’t take long and colonize rapidly.
My GS-jars for example took less than a week until I put them to substrate.
It mostly depends on the genetics, more specifically, the senescense. If the culture you got is a bit older, it takes longer to grow and is more prone to contam. This time I got lucky, but often, many growers “cut” their LCs, which results in too many cell divisions.
That’s why we work with culture slants. I don’t trust my supplier enough to give me fresh specimens, and therefore I try to get some of them fruiting, take spore prints to reset the genetics, and then grow from them. When I’ve done that, I back up the culture in my fridge until I need to use it again.
This guarantees me to get super low P-values, resulting in strong immunity and growth, which I strongly need, since I grow in unsterile environments.
Also, if something goes horribly wrong, I always have backups. I learned from my past mistakes…
I experimented with starting a culture of button mushrooms from stems. It worked well until I transferred it to the grain jars, but I’m planning to try and do the same with my oysters once I get the first batch. Gotta know how to keep them going!
Can you describe your process? Did you clone the stems to agar first or did you put them directly to spawn?
So that was a totally off-the-cuff experiment based on some light reading, and while I was waiting for other supplies to arrive. Basically I found some bulk mushrooms at the store which still have mycelium on the stems. I boiled some cut-up pieces of cardboard, mixed in the chopped stems with it, and put everything in a small plastic container where I had drilled a few air holes in the sides. A few days later I discovered buttons prefer manure to grow on but I kept it going anyway, and the mycelium actually grew!
So after about three weeks of growth I had received my oyster spawn and purchased rye and popcorn. These were boiled for 15-20 minutes, allowed to dry for a couple hours, then moved to quart jars and put in the pressure canner for 90 minutes. The next day I built a still air box and transferred the oyster spawn to several jars, and a couple days later I used the same process to transfer the button mushroom spawn to the remaining jars.
Well the oyster spawn did great, but apparently I made a big mistake somewhere with the button mushrooms because I have blue-green mold growing in both jars within a week.
If I decide to try growing the buttons again then my plan is put the stems directly into the grain jars so I can eliminate one step of transferring and reduce the risk of contamination. I’m thinking about using this method for the oysters as well. I’ve never done spore prints and don’t know anything about making agar, but starting off from pieces of stems sure seemed to work with the buttons.