This image was taken through the 100x oil objective and a 2x camera adapter projecting the image into a Nikon D7500. The sample is a leaf from one of my plants (Dioscorea elephantipes, but I don’t think this picture would look very different for other plant species)

The edges of he leaf were already yellowish brown. Here is a photo of that area with much less chlorophyll:

And here is a photo through the 40x objective using oblique illumination:

If you want to see some really fantastic photos of plant stomata I recommend having a look at Rolf Vossen’s photographs here: https://microscopyofnature.com/stomata

I am looking through his documentation trying to understand how he managed to get those images. They are spectacular.

  • SalamanderOPMA
    2·
    13 days ago

    Great album cover!!

    Is this with a filter? A stain? Post-processing?

      • SalamanderOPMA
        3·
        13 days ago

        Very cool. Before staining, did you prepare it in some way?

        • Doxatek
          3·
          13 days ago

          Yeah. I was supposed to clear the leaf of cellular contents so I could see through it to the other side. Accidentally left it in acid for too long and each layer separated. This is only the epidermis one later thick

          • SalamanderOPMA
            3·
            13 days ago

            Thanks for the explanation. I will look into how to remove cellular contents and how to separate layers. I didn’t know this could be achieved with acid :D