From the left to the right (in the attached photo): two jars of Pleurotus ostreatus spores (now mycelium) growing in grain, two jars of P. ostreatus liquid culture cloned from agar, and two jars of Pleurotus pulmonarius liquid culture cloned from agar.

I also have five agar plates I inoculated with a different collection of P. ostreatus spores, but there’s no visible growth yet–so I’ll leave those out.

The P. pulmonarius was cloned from a fruiting body growing on a commercial grower’s spent block that was salvaged from their waste pile. It’s actually a pretty happy, and fast-growing variety. It seems that it hasn’t reached senescence yet.

The P. ostreatus LC was cloned from a several-generations old sample that was initially from a liquid culture bought through amazon. It hasn’t performed super well–which is why I’ve taken it to spore in two different experiments. I may end up disposing of this LC, but I’ll probably store it in the fridge while I work on isolating some new strains from the spore experiments. Hopefully I’ll win the P. ostreatus lottery…

All of this is just done in a simple Still Air Box, it’s just a hobby for now. Some day I want to assemble a proper lab with a FFU/LFH, but that day is not today!

Anyone else culturing anything fun?

  • lightingnerd@lemmy.worldOPEnglish
    3·
    2 years ago

    Nice! Yeah, I mostly want it for convenience of working with agar–but the need just isn’t there yet. I’m considering a redneck version using one of the 4" thick MERV 12 filters, which are rated to filter 0.3um particles, just not 99.99% of them. I know for a fact it would be much better than my SAB in terms of contamination risk, and I’m considering building my own wooden impeller fan, so if I can scrap an old high RPM motor–I may be able to finagle a basic FFU together for under $50.

    • SalamanderMAEnglish
      2·
      2 years ago

      Cool! Looks like you’ve done your research :-)

      I still haven’t done a deep dive into it, but from the little I did read about flowhood design I understand that one needs to get some numbers right in the design to achieve the correct laminar flow. Do you happen to have a favorite resource on how to build a flowhood? Or are you creating your own design based on what you learned from multiple sources? If you do have a resource you can recommend, I’d love to look at it!