Ignore the chaos in the background. 😅 My dog came through and knocked everything over with the wiggles.

  • SalamanderA
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    1 year ago

    😱 Cool! This smells like the start of a new hobby…

    • Doxatek
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      1 year ago

      Haha good luck! I do tissue culture for a living. You definitely can do it at home but you have a lot of work cut out for you

      • SalamanderA
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        1 year ago

        Haha, thanks! I have a few years of experience with home mycology (agar work/sterile technique) and I also keep lots of houseplants, so I’m thinking some of these skills might transfer when I do get into it. A reason why I’m interested in this is because some of the work I’ve read about fungi-root interactions was performed on fungi grown on root tissue cultures - which makes sense - but I don’t really know how one would practically achieve that. So I’m very curious about it…

        • Doxatek
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          1 year ago

          Gotcha. While never having cultured fungus (on purpose) plants definitely pose unique challenges. Such as hormone application. I also think, though I don’t have extensive knowledge and fungal media composition, that the media for plants can need quite a lot more sugar which makes bacteria and fungi even more a problem.

          Really cool you’ve already done it at home though. Did you do pressure cooker method

          • SalamanderA
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            1 year ago

            Such as hormone application.

            Oh, I haven’t worked with hormones before. That would be interesting.

            Did you do pressure cooker method

            Yeah, my pressure cookers are basically my autoclaves. And I use a still-air box (30L transparent plastic tub with holes) for pouring plates and transfers.

            • Doxatek
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              1 year ago

              Oh yeah the hormones are cool you can make them do some weird stuff haha